BrdU-Hoechst-ethidium bromide (EB) quenching technique for studying kinetics of hematopoiesis.
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Photophysical Processes of Ethidium Bromide in Micelles and Reverse Micelles
Photophysical processes of ethidium bromide (EB) in homogeneous solutions, micelles, and reverse micelles have been investigated. In acetonitrile, the fluorescence intensity and lifetime of EB are 6.3 ( 0.3 times those in water and 1.25 ( 0.1 times those in acetone. This is attributed to the weaker hydrogen-bond acceptor property of acetonitrile, compared to water and acetone. Addition of water...
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Mitochondrial DNA (mtDNA) in tumor cells was found to play an important role in maintaining the malignant phenotype. Using laser scanning confocal fluorescence microscopy (LSCFM) in a recent work, we reported a variable fluorescence intensity of ethidium bromide (EB) in mitochondria nucleoids of living carcinoma cells. Since when EB is bound to nucleic acids its fluorescence is intensified; a h...
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Breaks are introduced into DNA strands when DNA solutions containing ethidium bromide (EB) are exposed to incandescent light. The nicking rate is sensitive to the concentration of EB and the light intensity. At short exposure times, this rate is limited by photon capture and formation of an intermediate capable of nicking DNA and zero-order nicking kinetics are observed. If the EB is pre-irradi...
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ورودعنوان ژورنال:
- Blood
دوره 80 1 شماره
صفحات -
تاریخ انتشار 1992